Noncontact Respiratory Measurement for Multiple People at Arbitrary Locations Using Array Radar and Respiratory-Space Clustering

We developed a noncontact measurement system for monitoring the respiration of multiple people using millimeter-wave array radar. To separate the radar echoes of multiple people, conventional techniques cluster the radar echoes in the time, frequency, or spatial domain. Focusing on the measurement of the respiratory signals of multiple people, we propose a method called respiratory-space clustering, in which individual differences in the respiratory rate are effectively exploited to accurately resolve the echoes from human bodies. The proposed respiratory-space clustering can separate echoes, even when people are located close to each other. In addition, the proposed method can be applied when the number of targets is unknown and can accurately estimate the number and positions of people. We perform multiple experiments involving five or seven participants to verify the performance of the proposed method, and quantitatively evaluate the estimation accuracy for the number of people and the respiratory intervals. The experimental results show that the average root-mean-square error in estimating the respiratory interval is 196 ms using the proposed method. The use of the proposed method, rather the conventional method, improves the accuracy of the estimation of the number of people by 85.0%, which indicates the effectiveness of the proposed method for the measurement of the respiration of multiple people

Radar-Based Automatic Detection of Sleep Apnea Using Support Vector Machine

2020 International Symposium on Antennas and Propagation (ISAP), 25-28 Jan. 2021, Osaka, JapanEarly diagnosis of sleep-apnea-related breathing problems helps to avoid the increased risk they can cause. In this study, we performed simultaneous radar measurements and polysomnography on patients with sleep apnea. A support vector machine algorithm was applied to the radar data to automatically detect sleep apnea events. Support vector machine parameters were optimized using the relationship between the radar and polysomnography data. The support vector machine was found to be effective in noncontact detection of central/mixed sleep apnea events using radar data. The proposed approach achieved an accuracy of 79.5%, a recall of 71.2%, and a precision of 71.2%

Legitimation of Income Inequality in Japan: A Comparison with South Korea and the United States

This paper was submitted to the Research Network Q “Asian Capitalisms” of the 2019 SASE annual conference in New York and won the 2019 FFJ/SASE Award for the Best Paper on Asia. This research was supported by JSPS KAKENHI Grant Number JP16H03688.Why does Japan have a large earnings gap between regular and non-regular employment? We attempt to answer this question through the lens of the legitimation of inequality, assuming the possibility that the inequality remains unresolved because it is legitimized due to institutional settings in Japanese society. In this paper, we explore to what extent the inequality is legitimized, as well as how it is legitimized, in Japanese society by analyzing data collected from a comparative vignette survey on the just income of fictitious workers in comparison with South Korea and the United States. The results of multilevel model analysis show that the income gap between regular and non-regular employees is accepted as just in Japan and South Korea. Moreover, non-regular worker respondents think that the income gap should be wider than regular worker respondents do (against their own interests) only in Japan. To some extent, the acceptance of income inequality and its over-acceptance by non-regular workers can be explained by the assumed difference in the duties and opportunities in the workplace between regular and non-regular employees, which is brought about by the male breadwinner model and Japanese firms’ practices to secure the livelihood of employees and their families. Based on the results, we argue that the income inequality between regular and non-regular employment in Japan has a stronger logic of legitimation and, therefore, is more likely to persist than in other countries

Noncontact Detection of Sleep Apnea Using Radar and Expectation-Maximization Algorithm

Sleep apnea syndrome requires early diagnosis because this syndrome can lead to a variety of health problems. If sleep apnea events can be detected in a noncontact manner using radar, we can then avoid the discomfort caused by the contact-type sensors that are used in conventional polysomnography. This study proposes a novel radar-based method for accurate detection of sleep apnea events. The proposed method uses the expectation-maximization algorithm to extract the respiratory features that form normal and abnormal breathing patterns, resulting in an adaptive apnea detection capability without any requirement for empirical parameters. We conducted an experimental quantitative evaluation of the proposed method by performing polysomnography and radar measurements simultaneously in five patients with the symptoms of sleep apnea syndrome. Through these experiments, we show that the proposed method can detect the number of apnea and hypopnea events per hour with an error of 4.8 times/hour; this represents an improvement in the accuracy by 1.8 times when compared with the conventional threshold-based method and demonstrates the effectiveness of our proposed method.Comment: 8 pages, 12 figures, 3 tables. This work is going to be submitted to the IEEE for possible publicatio

Involvement of resistin-like molecule β in the development of methionine-choline deficient diet-induced non-alcoholic steatohepatitis in mice

Resistin-like molecule β (RELMβ) reportedly has multiple functions including local immune responses in the gut. In this study, we investigated the possible contribution of RELMβ to non-alcoholic steatohepatitis (NASH) development. First, RELMβ knock-out (KO) mice were shown to be resistant to methionine-choline deficient (MCD) diet-induced NASH development. Since it was newly revealed that Kupffer cells in the liver express RELMβ and that RELMβ expression levels in the colon and the numbers of RELMβ-positive Kupffer cells were both increased in this model, we carried out further experiments using radiation chimeras between wild-type and RELMβ-KO mice to distinguish between the contributions of RELMβ in these two organs. These experiments revealed the requirement of RELMβ in both organs for full manifestation of NASH, while deletion of each one alone attenuated the development of NASH with reduced serum lipopolysaccharide (LPS) levels. The higher proportion of lactic acid bacteria in the gut microbiota of RELMβ-KO than in that of wild-type mice may be one of the mechanisms underlying the lower serum LPS level the former. These data suggest the contribution of increases in RELMβ in the gut and Kupffer cells to NASH development, raising the possibility of RELMβ being a novel therapeutic target for NASH

Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms

The murine peptidylarginine deiminase (PAD) has five isoforms encoded by different genes and partici- pates in a variety of cellular functions through the citrullination of target proteins. The crystal structure of human PAD4 with a dimeric form was previously solved because of the enzyme’s relevance to rheuma- toid arthritis. PAD6, abundant in mouse oocytes and eggs, is believed to take part in early events of embryogenesis, but its biochemical properties are little understood. Here we have purified and charac- terized a recombinant PAD6. A PAD6 cDNA was cloned from mouse ovary RNA and expressed in Escherichia coli through pET29 and pGEX vectors. When benzoyl-L-arginine ethyl ester was used as a substrate, no appreciable activity was detected with a cell homogenate under conditions where a human PAD4 cDNA caused significant activity. Both pro- teins were affinity-purified to near homogeneity. The circular dichroism spectra of PAD6 and human PAD4 were similar in the far ultraviolet region. On molecular sieving, PAD6 was eluted faster than human PAD4. The cross-linking of PAD6 with dime- thyl suberimidate clearly showed six bands on an sodium dodecyl sulfate-polyacrylamide gel. These results indicate that PAD6 can constitute a hexameric structure. The purified PAD6 still showed no enzy- matic activity. This unique structure and loss in enzymatic activity is strongly suggested to favor the formation of egg cytoplasmic sheets as the architectu- ral protein

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target

ハンシャキョウ オ モチイタ リアルタイム サンジゲン チョウオンパ イメージング システム ト ソノ テキヨウ

京都大学0048新制・課程博士博士(情報学)甲第13227号情博第271号新制||情||54(附属図書館)UT51-2007-H500京都大学大学院情報学研究科通信情報システム専攻(主査)教授 佐藤 亨, 教授 津田 敏隆, 教授 松田 哲也学位規則第4条第1項該当Doctor of InformaticsKyoto UniversityDFA